Mammalian Cell Culture (2013)

Description:
The purpose of this course is to introduce participants to basic mammalian cell culture techniques. The course will provide essential information on the aseptic techniques, and passaging of adherent and non-adherent cells. During the course, primary cell cultures will be established, and the differences between continuous cell lines and primary cells will be discussed. Finally, basic methods for monitoring cell growth and viability will be performed. The course will be divided between lectures, demonstrations, and laboratory exercises. The exercises will cover the topics included in the theoretical part of the course.

Topics
• Aseptic techniques in cell culturing
• Cell freezing, thawing, and passaging
• Assays for cell cycle and proliferation
• Establishment of primary cell culture
• Introduction to contrasting microscopic techniques
• Basic principles of image analysis

Literature
Junqueira & Carneiro: Basic Histology, chapters 2 and 3 or equivalent

Organizer:
Associate Professor Trine Fink, email: trinef@hst.aau.dk
Professor Vladimir Zachar, email: vlaz@hst.aau.dk

Lecturers:
Associate Professor Trine Fink and Professor Vladimir Zachar, Aalborg University

ECTS:
1.5

Time:
June 12 and 13, 2013 (8.15-16.00)

Place:
Aalborg University
Fredrik Bajers Vej 7E, Room E3-209

Zip code:
9220

City:
Aalborg

Number of seats:

Deadline:
May 15, 2013

Important information concerning PhD courses
We have over some time experienced problems with no-show for both project and general courses. It has now reached a point where we are forced to take action. Therefore, the Doctoral School has decided to introduce a no-show fee of DKK 5,000 for each course where the student does not show up. Cancellations are accepted no later than 2 weeks before start of the course. Registered illness is of course an acceptable reason for not showing up on those days. Furthermore, all courses open for registration approximately three months before start. This can hopefully also provide new students a chance to register for courses during the year. We look forward to your registrations.