Couse name: Advanced characterization of Extracellular Vesicles
PhD Programme: Clinical Science, Laboratory and Translational Medicine
Description:
Aim: To provide theoretical knowledge on extracellular vesicles (EV), and hands-on experience with advanced methods for molecular characterization.
Content: In recent years, EVs have been recognized to play an important role in health and disease by trafficking biomolecules between cells. Apart from being involved in maintaining homeostatic processes in a healthy organism, EVs have also been found to be important in disease progression since both diseased cells and pathogens release EVs to facilitate their survival. This suggests that EVs can be used as diagnostic markers for disease conditions. Furthermore, the therapeutic potential of EVs and their use as vaccines are currently being explored all underpinning the prospects of EV-research.
In this course, we will cover basic aspects of EVs, and explore some of the methods using antibody-based technologies for their characterization. A part of the course will include antibody and molecular binding theory. In addition, the course will focus on techniques used for broad biomarker screening followed by more targeted approaches.
As it is not a trivial task to work with EVs, we will discuss critical aspects to consider during collection and isolation of EVs from various sample types and how to perform advanced characterization of EVs.
During the practical part, you will learn how to perform high-sensitive flow cytometry and EV Array. The size distribution and number of EVs in the samples will be examined by fluorescent nanoparticle tracking analysis.
Organizer: Prof. Aase Handberg, email: aaha@rn.dk; Senior Scientist, associate professor Malene Møller Jørgensen; email: maljoe@rn.dk
Lecturers: Aase Handberg, Malene M Jørgensen, Maiken Mellergaard, Rikke Bæk and invited speakers.
ECTS: 3.0
Dates: 6.-10. November 2023
Time: 8:30 – 15:30
Place: Dep. Clinical Immunology, and Forskningens hus, Aalborg University Hospital
Zip code: 9000
City: Aalborg
Number of seats: 10
Deadline: October 15
Important information concerning PhD courses:
Literature/Requirements
The students participating to this course should have basic knowledge on extracellular vesicles and their nomenclature and biogenesis.
Literature: A reading list with relevant papers and books chapters will be announced before the course.
Important information concerning PhD courses:
We have over some time experienced problems with no-show for both project and general courses. It has now reached a point where we are forced to take action. Therefore, the Doctoral School has decided to introduce a no-show fee of DKK 3.000 for each course where the student does not show up. Cancellations are accepted no later than 2 weeks before start of the course. Registered illness is of course an acceptable reason for not showing up on those days. Furthermore, all courses open for registration approximately four months before start. This can hopefully also provide new students a chance to register for courses during the year. We look forward to your registrations.
- Teacher: Aase Handberg
- Teacher: Malene Jørgensen